硝酸纤维素膜2片。硝酸纤维素膜尺寸： 7× 8.5cm，孔径：0.2μm，结合能力： 90 μg/cm2。Nitrocellulose Sandwiches是预包装的sandwiches，每包包含一张预切好的硝酸纤维素膜和2张0.34mm厚的印迹纸。为了提供便利和节省时间，已经用CST的western blot方案进行了验证，并且CST的科研工作者也首选此产品。Nitrocellulose Sandwiches是结合不同分子量的蛋白的理想产品。0.2 μm的孔径使低分子量的蛋白也能被高度保留下来，甚至是低于20kDa的蛋白。Nitrocellulose Sandwiches对于大多数常用的转印方法都适用，包括槽、半干和真空印迹技术。本产品还适用于色度法、荧光和化学发光检测。对于化学发光检测，CST推荐基于HRP的系统。与PVDF膜不一样，硝酸纤维素不需要甲醇预先浸湿这个步骤。 Nitrocellulose Sandwiches，2 Pack. Dimensions: 7 X 8.5 cm, Pore Size: 0.2 μm, Binding Capacity: 90 μg/cm2. Pre-packaged sandwiches (20/pack) consisting of a precut nitrocellulose membrane and 2 sheets of 0.34 mm thick blotting paper. Intended to provide convenience and time savings, they are also validated for use in CST's Western Blot protocol and are the preferred membrane used by Cell Signaling Technology scientists. Nitrocellulose Sandwiches are ideal for binding proteins of all molecular weights; the 0.2 μm pore size ensures high retention of low molecular weight proteins, even below 20 kDa. Nitrocellulose Sandwiches are suitable for most common transfer methods including tank, semi-dry, and vacuum blotting. This product is also suitable for colorimetric, fluorescent, and chemiluminescent detection. For chemiluminescent detection, CST recommends HRP based systems. Unlike PVDF, nitrocellulose does not require a methanol pre-wetting step.
硝酸纤维素膜20片。硝酸纤维素膜尺寸： 7× 8.5cm，孔径：0.2μm，结合能力： 90 μg/cm2。Nitrocellulose Sandwiches是预包装的sandwiches，每包包含一张预切好的硝酸纤维素膜和2张0.34mm厚的印迹纸。为了提供便利和节省时间，已经用CST的western blot方案进行了验证，并且CST的科研工作者也首选此产品。Nitrocellulose Sandwiches是结合不同分子量的蛋白的理想产品。0.2 μm的孔径使低分子量的蛋白也能被高度保留下来，甚至是低于20kDa的蛋白。Nitrocellulose Sandwiches对于大多数常用的转印方法都适用，包括槽、半干和真空印迹技术。本产品还适用于色度法、荧光和化学发光检测。对于化学发光检测，CST推荐基于HRP的系统。与PVDF膜不一样，硝酸纤维素不需要甲醇预先浸湿这个步骤。 Nitrocellulose Sandwiches，20 Pack. Dimensions: 7 X 8.5 cm, Pore Size: 0.2 μm, Binding Capacity: 90 μg/cm2. Pre-packaged sandwiches (20/pack) consisting of a precut nitrocellulose membrane and 2 sheets of 0.34 mm thick blotting paper. Intended to provide convenience and time savings, they are also validated for use in CST's Western Blot protocol and are the preferred membrane used by Cell Signaling Technology scientists. Nitrocellulose Sandwiches are ideal for binding proteins of all molecular weights; the 0.2 μm pore size ensures high retention of low molecular weight proteins, even below 20 kDa. Nitrocellulose Sandwiches are suitable for most common transfer methods including tank, semi-dry, and vacuum blotting. This product is also suitable for colorimetric, fluorescent, and chemiluminescent detection. For chemiluminescent detection, CST recommends HRP based systems. Unlike PVDF, nitrocellulose does not require a methanol pre-wetting step.
强化硝酸纤维素膜，内带支撑介质，双面均匀覆盖纯硝酸纤维素。强韧不脆，有效防止撕裂皱缩。孔径0.45，结合载量 100 µg/cm2 200 x 200 mmThe classical transfer medium for binding proteins and nucleic acids - the supporting tissue prevents breaking, tearing, or shrinking Membrane material 100 % nitrocellulose with inert supporting tissue Membrane character Hydrophilic Pore size 0.45 µm Binding capacity 100 µg/cm2 Applications • Southern, Northern, and Western blotting, colony and plaque transfer, dot blotting• Also available without supporting tissue, see Porablot NCP nitrocellulose membrane with supporting tissue, pore size: 0.45 µm, size: 200 x 200 mm pack of 10 sheets
强化硝酸纤维素膜，内带支撑介质，双面均匀覆盖纯硝酸纤维素。强韧不脆，有效防止撕裂皱缩。孔径0.45，结合载量 100 µg/cm2 0.3 x 3 mThe classical transfer medium for binding proteins and nucleic acids - the supporting tissue prevents breaking, tearing, or shrinking Membrane material 100 % nitrocellulose with inert supporting tissue Membrane character Hydrophilic Pore size 0.45 µm Binding capacity 100 µg/cm2 Applications • Southern, Northern, and Western blotting, colony and plaque transfer, dot blotting• Also available without supporting tissue, see Porablot NCP nitrocellulose membrane with supporting tissue, pore size: 0.45 µm, size: 0.3 x 3 m pack of 1 roll__UN 3270 Nitrocellulose membrane filters 4.1 II 0.32 Kg ADR/GGVSEB F1LQ 8 PAX 458 1 Kg CAO 458
经典纯硝酸纤维素膜，孔径0.45 µm，载量100 µg/cm2 0.3 x 3 mThe classical Nitrocellulose membranes for binding proteins and nucleic acids Membrane material 100 % nitrocellulose Membrane character Hydrophilic Pore size 0.45 µm Binding capacity 100 µg/cm2 Applications • Southern, Northern, and Western blotting, colony and plaque transfer, dot blotting• Also available with a supporting tissue, see Porablot NCL Membrane filters of high purity nitrocellulose are the classical transfer medium for binding proteins and nucleic acids. The range of application includes Southern, Northern and Western blotting as well as colony, plaque transfer and dot blotting. Although the binding mechanism is not understood in all details, it is assumed that the macromolecules are bound via hydrophobic interaction and hydrogen bonding. Porablot NCP membranes consist of 100 % nitrocellulose and are supplied with a pore size of 0.45 μm. With a binding capacity of 100 μg/cm2 this membrane is well suited for protein blotting. The hydrophilic character of this membrane ensures good contact with the gel during botting. Additionally, nitrocellulose can be easily blocked, for example with casein or albumin. Due to the low background, all conventional detection methods, such as antigen/antibody, autoradiography, biotin labelling and chemiluminescence, yield good results. nitrocellulose membrane, pore size: 0.45 µm, size: 0.3 x 3 m pack of 1 roll __UN 3270 Nitrocellulose membrane filters 4.1 II 0.32 Kg ADR/GGVSEB F1 LQ 8 PAX 458 1 Kg CAO 458 15 Kg Q: 0.32 / 0.02
经典的PVDF膜，高强度，不易撕裂，耐受多数有机溶剂，甲醇处理不收缩。孔径0.2um，结合载量50-100 µg/cm2The classical transfer membrane for Western transfer, protein binding assays, and protein sequencing Membrane material Polyvinylidene difluoride (PVDF) membrane character Hydrophobic Pore size: 0.20 µmBinding capacity: 50-100 µg/cm2* * For large, globular proteins such as immunoglobulins. For smaller peptides the binding capacity is correspondingly larger Applications • Western blotting, protein binding assays, protein sequencing This polyvinylidene difluoride (PVDF) membrane is especially suited for Western transfer, protein binding assays and protein sequencing. The pure white microporous surface (pore size 0.2 μm) can bind proteins of very different type via hydrophobic interaction. The proteins thus immobilised can directly be used for gas or liquid phase sequencing or amino acid analyses. Porablot PVDF has a binding capacity of 50 to 100 μg/cm2 for large, globular proteins such as immunoglobins. For smaller peptides it shows a correspondingly larger binding capacity. Non-specific interactions with the membrane surface can be avoided with a number of blocking reagents, such as casein, bovine serum albumin and detergents. In addition to anionic dyes, gold or silver staining, detection of proteins can also be performed with non-radioactive detection systems (e.g., biotin/avidin peroxidase system). The Porablot PVDF membrane features a high tensile strength and tear resistance. It is resistant towards most organic solvents. There is no risk of shrinking upon treatment with methanol. Since even trifluoroacetic acid and triethylamine do not attack the surface, the membranes are suitable for use in amino acid analysers and gas phase sequencing instruments. PVDF membrane, pore size: 0.2 µm, size: 0.25 x 3 m pack of 1 roll
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