Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 μg DOTAP per μg DNA and approximately 10-20 μg DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.
Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 μg DOTAP per μg DNA and approximately 10-20 μg DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.
Suitable for transient and stable transfection of nucleic acids into primary neurons and cultured neuronal cell lines. Use approximately 15-120 mul Neuroporter(TM) Transfection Reagent and 6-8 mug DNA (in provided unique DNA Dilution buffer when required) per 6 cm cell culture plate. The following cells have been successfully transfected using the Neuroporter(TM) Transfection Kit: . C6 glioma (human). Cortical neurons (rat primary). Dorsal Root Ganglion (DRG) cells (rat). NT2 neurons(human precursor cells). NT neurons (human differentiated cells). Subventricular Zone (SVZ) cells (mouse). White matter cells (mouse)
20 µg 纯化的即用型的质粒DNA，更多可供20次转染Enables highly efficient DNA transfection in a variety of cell lines including CHO-K1, COS, LNCaP, NIH/3T3, 293, T24, C2C12, SF-9, primary human keratinocytes, primary aortic smooth muscle, primary rabbit myoblasts and human bone marrow endothelial cells (HBMEC)Recommended for use with CRISPR/Cas9 KO Plasmids, HDR Plasmids and Cre Vectorstore at 4° C; DO NOT FREEZE; protect from light
1 ml; 10mg/ml in sterile waterhighly efficient infection reagent used to introduce retroviral vectors into mammalian cellsalso known as hexamethrine bromide, 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromidePolybrene® is a registered trademark of Abbott Laboratories Corp.Store at -20º C
20 mlReduced-serum medium suitable for transfections with DNA PlasmidsRecommended for use with Plasmid Transfection Reagent (sc-108061) and suspension of DNA for transfection including: shRNA Plasmids, CRISPR/Cas9 KO Plasmids, HDR Plasmids or Cre VectorModification of Eagle's Minimal Essential Medium, buffered with HEPES and sodium bicarbonate, and supplemented with hypoxanthine, thymidine, sodium pyruvate, L-glutamine, trace elements and growth factors; protein level is minimal with insulin and transferrin being the only protein supplements; phenol red included at a reduced concentration as a pH indicatorStore at 4° C; light sensitive
0.2 ml; Sufficient for 50-100 transfections (per well in a 6-well plate)Enables highly efficient DNA transfection in a variety of cell lines including CHO-K1, COS, LNCaP, NIH/3T3, 293, T24, C2C12, SF-9, primary human keratinocytes, primary aortic smooth muscle, primary rabbit myoblasts and human bone marrow endothelial cells (HBMEC)Recommended for use with shRNA PlasmidsEffective in both serum-containing medium and serum-free medium, offering high efficiencies with minimal cytotoxicitystore at 4° C; DO NOT FREEZE; protect from light
0.3 ml; sufficient for 50-100 transfections (using 12-well plates)enables highly efficient siRNA transfection in a variety of cell lines including HeLa, A549, Jurkat and NIH/3T3Effective in both serum-containing medium and serum-free medium, offering high efficiencies with minimal cytotoxicitystore at 4° C; do not freeze; protect from light
20 mlreduced-serum medium suitable for addition to siRNA suspension and siRNA transfection reagent, immediately prior to cell transfectionModification of Eagle's Minimal Essential Medium, buffered with HEPES and sodium bicarbonate, and supplemented with hypoxanthine, thymidine, sodium pyruvate, L-glutamine, trace elements and growth factors; protein level is minimal with insulin and transferrin being the only protein supplements; phenol red included at a reduced concentration as a pH indicatorstore at 4° C; light sensitive
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