Blood direct PCR Kit可以直接对全血样本进行PCR,无需进行DNA纯化或样品预处理。可用于直接扩增的血样类型包括:新鲜血液、4℃贮存血液、冷冻血
液以及储存在Whatman903®和FTA®商用卡上的干血渍,且兼容所有常规抗凝剂(EDTA、柠檬酸盐、肝素等)。试剂盒中包含Phanta Blood Super-Fidelity DNA Polymerase,是在Phanta Super-Fidelity DNA Polymerase基础上经过工程学改造而成的。该酶对全血样品中的PCR抑制剂具有超强的抵抗力,可扩增全血浓度高达40%。配以精心优化的2 × Phanta Blood Buffer V2,Blood Direct PCR Kit V2能够从全血样品中高效扩增长达10 kb的基因组片段。Phanta Blood Super-Fidelity DNA Polymerase扩增产物为平端,适用于ClonExpress®快速克隆试剂盒(C112/C113)。试剂盒配有与哺乳动物和其他许多脊椎动物兼容的引物预混液Positive control primer mix,可用于进行阳性对照反应。
Blood direct PCR Kit可以直接对全血样本进行PCR,无需进行DNA纯化或样品预处理。可用于直接扩增的血样类型包括:新鲜血液、4℃贮存血液、冷冻血
液以及储存在Whatman903®和FTA®商用卡上的干血渍,且兼容所有常规抗凝剂(EDTA、柠檬酸盐、肝素等)。试剂盒中包含Phanta Blood Super-Fidelity DNA Polymerase,是在Phanta Super-Fidelity DNA Polymerase基础上经过工程学改造而成的。该酶对全血样品中的PCR抑制剂具有超强的抵抗力,可扩增全血浓度高达40%。配以精心优化的2 × Phanta Blood Buffer V2,Blood Direct PCR Kit V2能够从全血样品中高效扩增长达10 kb的基因组片段。Phanta Blood Super-Fidelity DNA Polymerase扩增产物为平端,适用于ClonExpress®快速克隆试剂盒(C112/C113)。试剂盒配有与哺乳动物和其他许多脊椎动物兼容的引物预混液Positive control primer mix,可用于进行阳性对照反应。
ExcelTaq Blood Direct DNA Polymerase, 5 U/ μl, 500 U x 1 ExcelTaq全血专用DNA聚合酶,可耐受血液样本中的抑制物,能直接从血液样本中,扩增放大其目标DNA省去繁重萃取DNA之步骤。400 Rxn. highly tolerant in the presence of PCR interfering/inhibiting substances in blood, ExcelTaq Blood Direct DNA polymerase is designed for amplifying target DNA directly from whole blood, eliminating the need for a lengthy DNA isolation process.
ExcelTaq 5X Blood Direct PCR Master Mix Kit, 200 Rxn ExcelTaq全血专用5XPCR预混试剂组,200 Rxn, 含5X预混试剂组缓冲液、dNTPs、ExcelTaq全血专用DNA聚合酶、OrangeG、甘油等。能直接从血液样本中,扩增放大其目标DNA。除引物和DNA模板(血液)需使用者自行添加外,包含PCR反应所有的必要组分。预混试剂中含有电泳追踪染液,可扩增后直接电泳分析。产品特点:1,具有5'-3'DNA聚合酶活性,无3'-5'核酸外切酶活性。2,可直接进行血液样本快速扩增,适用于高通量PCR。3,重复性高,减少操作误差。4,PCR产物可直接上样,方便后续电泳分析。5,含有一对阳性对照引子对,可适用于灵长类动物血液样本扩增。For amplifying target DNA directly from whole blood. Therefore eliminating the need for a lengthy DNA isolation process. The ExcelTaq Blood Direct PCR Master Mix contains a unique blend of 5× concentrated mixture of all the essential components of a PCR reaction. The ExcelTaq Blood Direct PCR Master Mix also contains the PCR friendly loading tracking dye solution Orange G. This inclusion of Orange G tracking dye allows for a more convenient post-PCR gel electrophoresis analysis. The ExcelTaq 5x Blood Direct PCR Master Mix is capable of tolerating the presence of PCR interfering/inhibiting substances in blood. The ExcelTaq 5x Blood Direct PCR Master Mix is ideal for high-throughput screening of blood samples for high reproducibility. The ExcelTaq 5x Blood Direct DNA polymerase includes a pair of positive control primers (CCR5) that are compatible with mammalian blood samples.
The ExcelTaq 5× Blood Direct PCR Master Mix Kit includes a pairs of positive control primers (CCR5) that are compatible with primate blood sample.