识别位点GTGCT/COne unit is defined as the amount of enzyme required to convert 1 μg of supercoiled pUC19 DNA to open circular form in 1 hour at 37°C in a total reaction volume of 50 μl.
识别位点GTGCT/COne unit is defined as the amount of enzyme required to convert 1 μg of supercoiled pUC19 DNA to open circular form in 1 hour at 37°C in a total reaction volume of 50 μl.
Product Source
An E. coli strain that carries the cloned and modified BsiWI gene from Bacillus species (D. Clark). Unit Definition
One unit is defined as the amount of enzyme required to digest 1 μg of ΦX174 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Product Source
An E. coli strain that carries the cloned and modified BsiWI gene from Bacillus species (D. Clark). Unit Definition
One unit is defined as the amount of enzyme required to digest 1 μg of ΦX174 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in our video library, https://www.neb.com/tools-and-resources/video-library). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15–40 minutes.
The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in our video library, https://www.neb.com/tools-and-resources/video-library). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15–40 minutes.
识别位点GTA/TAC Categories: High-Fidelity (HF®) Restriction Endonucleases, Restriction Endonucleases: B, Time-Saver™ Qualified Restriction Enzymes；NEB extensively performs quality controls on all standard and high-fidelity (HF®) restriction enzymes. Product Source：An E. coli strain that carries the cloned and modified BstZ17I gene from Bacillus stearothermophilus 38M (Z. Chen).
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