从组织和细胞中快速提取6-700ug的更完整的RNA。离心柱式，载量达700ug。提供NucleoSpin® Filters Midi (shredders)有效降低裂解液粘稠度以均质化。提供rDNase实现纯化柱上的DNA降解，有效去除DNA污染。Midi spin kit for the isolation of RNA of highest integrity • Efficient removal of genomic DNA – rDNase included for on-column digestion*• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters Midi (shredders) included• Up to 600 μg ready-to-use RNA Technology: Silica-membrane technology Format: Midi spin columns Sample material: Fragment size: 200 b Typical yield: 180 µg from 10(7) HeLa cells, 620 µg from 4 x 10(7) HeLa cellsA260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 Elution volume: 500 µL Preparation time: 80 min/4 preps Binding capacity: 700 µg * The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA Midi system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:– the number of DNA copies per preparation: single copy target – decreasing PCR amplicon sizeNucleoSpin® RNA Midi Columns with Collection Tubes(15 mL), Collection Tubes (15 mL), NucleoSpin® Filters Midi, buffers, RNase-free rDNase 20 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA L Columns, NucleoSpin RNA L Filters, Elution Tubes, buffers, RNase-free rDNase

通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化，柱上进行DNA消化可有效去除DNA污染，还可平行纯化基因组DNA。柱载量200ug，洗脱体积40-120ul，适用于组织，培养细胞，细菌和酵母等Mini spin kit for the isolation of RNA of highest integrity • Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included• Up to 70 μg ready-to-use RNA• Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set• Also available for QIAcube® Technology: Silica-membrane technology Format: Mini spin columns Sample material: Fragment size: > 200 b Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cellsA260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 Elution volume: 40–120 µL Preparation time: 30 min/6 preps Binding capacity: 200 µg Applications** • Total RNA isolation from cultured cells, tissue (standard protocol) • Support protocol for total RNA from • Support protocol for total RNA from ≤ 100 μL biological fluids• Support protocol for RNA clean-up from reaction mixtures• Support protocol for total RNA from samples stored in RNAlater® • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays * The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:– the number of DNA copies per preparation: single copy target – decreasing PCR amplicon sizeNucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 50 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化，柱上进行DNA消化可有效去除DNA污染，还可平行纯化基因组DNA。柱载量200ug，洗脱体积40-120ul，适用于组织，培养细胞，细菌和酵母等Mini spin kit for the isolation of RNA of highest integrity • Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included• Up to 70 μg ready-to-use RNA• Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set• Also available for QIAcube® Technology: Silica-membrane technology Format: Mini spin columns Sample material: Fragment size: > 200 b Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cellsA260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 Elution volume: 40–120 µL Preparation time: 30 min/6 preps Binding capacity: 200 µg Applications** • Total RNA isolation from cultured cells, tissue (standard protocol) • Support protocol for total RNA from • Support protocol for total RNA from ≤ 100 μL biological fluids• Support protocol for RNA clean-up from reaction mixtures• Support protocol for total RNA from samples stored in RNAlater® • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays * The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:– the number of DNA copies per preparation: single copy target – decreasing PCR amplicon sizeNucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 250 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化，柱上进行DNA消化可有效去除DNA污染，还可平行纯化基因组DNA。柱载量200ug，洗脱体积40-120ul，适用于组织，培养细胞，细菌和酵母等Mini spin kit for the isolation of RNA of highest integrity • Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included• Up to 70 μg ready-to-use RNA• Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set• Also available for QIAcube® Technology: Silica-membrane technology Format: Mini spin columns Sample material: Fragment size: > 200 b Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cellsA260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 Elution volume: 40–120 µL Preparation time: 30 min/6 preps Binding capacity: 200 µg Applications** • Total RNA isolation from cultured cells, tissue (standard protocol) • Support protocol for total RNA from • Support protocol for total RNA from ≤ 100 μL biological fluids• Support protocol for RNA clean-up from reaction mixtures• Support protocol for total RNA from samples stored in RNAlater® • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays * The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:– the number of DNA copies per preparation: single copy target – decreasing PCR amplicon sizeNucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 10 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase