SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining.

马心肌红蛋白已被用作双向凝胶电泳的 pI 标记物。

来源：chicken egg white

使用“白蛋白选择指南”为您的应用选择正确的白蛋白

ProteoExtract 磷酸肽 TiO2 富集试剂盒是通过高选择性氧化钛固相富集复杂样品中磷酸化肽的有用工具。它利用新型的二氧化钛材料增强了常规识别和表征复杂蛋白混合物中大量磷酸化物质的能力。捕获和洗脱的磷酸肽级分可用于 MALDI 和 ESI 质谱分析。

GeneTech三色预染蛋白marker，蛋白条带从10Kd至180Kd，覆盖常见蛋白大小。 • 条带分布符合大部分科研工作者实验习惯 • 10种重组蛋白构成10K-180K蛋白分子量检测范围 • 无需稀释，无需煮沸、无需添加还原剂 货号 R1001-001 预染蛋白Ladder（10-180KDa）1x250ul（1支装） R1001-002 预染蛋白Ladder（10-180KDa）2x250ul（2支装） R1001-010 预染蛋白Ladder（10-180KDa）10x250ul（10支装） 产品描述 这是一款即用型预染三色蛋白Ladder，由10种分子量从10K-180K的重组蛋白组成。预染后的蛋白分子量相对于标准分子量变化在5%左右，在<10%的低浓度胶中，Ladder 中低分子量的蛋白会随着染料往前迁移。在不同的SDS-PAGE 缓冲系统中，预染Ladder中蛋白迁移速率会有所不同，但是在同一缓冲系统中，用未预染Ladder校准，其偏差是差不多的。Western Blotting 实验中Ladder中分子量大的蛋白（＞100K）在转膜时需要较长的转移时间或者较大的转移电压。 储存条件 -20℃可保存1年以上，4℃保存3个月以上 建议使用量 Mini-gel：5μl/孔（0.75-1.0mm厚度）或者10μl/孔（1.5mm厚度） Large-gel：10μl/孔（0.75-1.0mm厚度）或者20μl/孔（1.5mm厚度） 注意事项 1、 室温解冻，不可加热 2、 使用前轻柔混合均匀 3、仅供科研使用

GeneTech三色预染蛋白marker，蛋白条带从10Kd至180Kd，覆盖常见蛋白大小。 • 条带分布符合大部分科研工作者实验习惯 • 10种重组蛋白构成10K-180K蛋白分子量检测范围 • 无需稀释，无需煮沸、无需添加还原剂 货号 R1001-001 预染蛋白Ladder（10-180KDa）1x250ul（1支装） R1001-002 预染蛋白Ladder（10-180KDa）2x250ul（2支装） R1001-010 预染蛋白Ladder（10-180KDa）10x250ul（10支装） 产品描述 这是一款即用型预染三色蛋白Ladder，由10种分子量从10K-180K的重组蛋白组成。预染后的蛋白分子量相对于标准分子量变化在5%左右，在<10%的低浓度胶中，Ladder 中低分子量的蛋白会随着染料往前迁移。在不同的SDS-PAGE 缓冲系统中，预染Ladder中蛋白迁移速率会有所不同，但是在同一缓冲系统中，用未预染Ladder校准，其偏差是差不多的。Western Blotting 实验中Ladder中分子量大的蛋白（＞100K）在转膜时需要较长的转移时间或者较大的转移电压。 储存条件 -20℃可保存1年以上，4℃保存3个月以上 建议使用量 Mini-gel：5μl/孔（0.75-1.0mm厚度）或者10μl/孔（1.5mm厚度） Large-gel：10μl/孔（0.75-1.0mm厚度）或者20μl/孔（1.5mm厚度） 注意事项 1、 室温解冻，不可加热 2、 使用前轻柔混合均匀 3、仅供科研使用

YesBlot™ Western Marker I包含重组蛋白IGg结合蛋白、甘油、SDS和示踪染料、Tris-HCl缓冲液。10条Ig结合蛋白可免疫检测，额外添加4条预染Marker可指示电泳进度和转膜进度，方便好用YesBlot™ Western Marker I is a ready-to-use mixture with ten IgG-binding proteins covering a wide range of molecular weights from 15 to 200 kDa in TrisGlycine buffer. YesBlot™ Western Marker I performs dual functions. First, it contains 4 pre-stained proteins (10, 25, 45 and 70 kDa) for monitoring protein separation during SDSPAGE, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the protein size. Second, ten IgGbinding proteins can be immuno-detected on film or by CCD imaging. YesBlot™ Western Marker I is compatible for chemiluminescent, fluorescent, chromogenic or other detection systems. In addition, YesBlot™ Western Marker I has two reference bands with enhanced intensity (at 30 kDa and 80 kDa). The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.

化学发光蛋白Marker

ExcelBand 3-color Pre-stained Protein Ladder Broad Range, 250 μl x 2 ExcelBand三色预染型广范围蛋白质分子量标记，即用型产品，内含15条预染蛋白条带，经电泳分离后分别呈现蓝色、绿色和红色，条带分明，清晰可见，其中蓝色增强条带为20kD、绿色条带为40KDa、红色条带为75KDa。片段范围：5-245KDa。4℃保存不小于3个月，-20℃保存不小于24个月。A ready-to-use three-color protein standard with 15 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine Buffer (3.5 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5200 3-color Pre-Stained Protein Ladder Broad Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

ExcelBand 3-color Pre-stained Protein Ladder Regular Range, 250 μl x 2 ExcelBand三色预染型常规范围蛋白质分子量标记，即用型产品，内含13条预染蛋白条带，经电泳分离后分别呈现蓝色、绿色和红色，条带分明，清晰可见，其中蓝色增强条带为20kD、绿色条带为40KDa、红色条带为75KDa。片段范围：10-180KDa。4℃保存不小于3个月，-20℃保存不小于24个月。A ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5000 ExcelBand 3-color Pre-Stained Protein Ladder Regular Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

ExcelBand 3-color Broad Range Protein Marker, 250 μl x 2 ExcelBand三彩蛋白质广范围分子量标记，即用型产品，内含13条预染蛋白条带，经电泳分离后分别呈现蓝色、绿色和红色，条带分明，清晰可见，其中绿色条带为25KDa、红色条带为75KDa。片段范围：5-245KDa。4℃保存不小于3个月，-20℃保存不小于24个月。A ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa, respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2700 ExcelBand 3-color Broad Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

ExcelBand 3-color High Range Protein Marker, 250 μl x 2 ExcelBand三彩蛋白质高分子量标记，即用型产品，内含12条预染蛋白条带，经电泳分离后分别呈现蓝色、绿色和红色，条带分明，清晰可见，其中绿色条带为25KDa、红色条带为75KDa。片段范围：10-245KDa。4℃保存不小于3个月，-20℃保存不小于24个月。ExcelBand 3-color High Range Protein Marker is a ready-to-use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2600 3-color Pre-Stained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

ExcelBand 3-color Regular Range Protein Marker, 250 μl x 2 ExcelBand 三彩预染型蛋白质常规分子量标记，即用型产品，内含10条预染蛋白条带，经电泳分离后分别呈现蓝色、绿色和红色，条带分明，清晰可见，其中绿色条带为25KDa、红色条带为75KDa。片段范围：10-180KDa。4℃保存不小于3个月，-20℃保存不小于24个月。A ready-to-use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 180 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). PM2500 ExcelBand 3-color Regular Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

ExcelBand Pink Blue Protein Marker, 250 μl x 2 ExcelBand粉蓝双色蛋白质分子量标记，即用型产品，用于15%蛋白质电泳凝胶分析，内含11条预染蛋白质条带，条带分明，清晰可见，其中有三条蓝色辨别参考条带，分别为10KDa、40KDa和90KDa。片段范围：10-165KDa。4℃保存不小于3个月，-20℃保存不小于24个月。two-color protein standard with 11 pre-stained proteins covering a wide range of molecular weights from 10 to 175 kDa in Tris-Glycine buffer (10 to 165 kDa in Bis-Tris (MOPS) buffer and 7 to 165 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a pink chromophore except for three reference bands of blue color (at 10, 40, and 90 kDa, respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2400 ExcelBand Pink Blue Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

ExcelBand All Blue Broad Range Protein Marker, 250 μl x 2 ExcelBand 湛蓝蛋白质广范围蛋白分子量标记，即用型产品，用于15%蛋白质电泳凝胶分析，内含12条预染蓝色之蛋白质条带，条带分明，清晰可见，其中有两条加量参考条带，分别为25KDa和72KDa。片段范围：10-240KDa。4℃保存不小于3个月，-20℃保存不小于24个月。A blue protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 240 kDa in Tris-Glycine buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-Glycine buffer). The PM1700 ExcelBand All Blue Broad Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

ExcelBand All Blue Regular Range Plus Protein Marker, 250 μl x 2 ExcelBand 湛蓝蛋白质常规加强分子量标记，即用型产品，用于15%蛋白质电泳凝胶分析，内含11条预染蓝色之蛋白质条带，条带分明，清晰可见，其中有两条加量参考条带，分别为25KDa和72KDa。片段范围：10-180KDa。4℃保存不小于3个月，-20℃保存不小于24个月。 A blue protein standard with 11 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-Glycine buffer). The PM1600 ExcelBand All Blue Regular Range Plus Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

ExcelBand 3-color Pre-stained Protein Ladder High Range, 250 μl x 2 ExcelBand 三彩预染型蛋白质常规分子量标记，高范围即用型产品，内含14条预染蛋白条带，经电泳分离后分别呈现蓝色、绿色和红色，条带分明，清晰可见，其中蓝色蛋白加量条带为20KDa、绿色条带为40KDa、红色条带为75KDa。片段范围：10-245KDa。4℃保存不小于3个月，-20℃保存不小于24个月。A ready-to-use three-color protein standard with 14 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5100 3-color Pre-Stained Protein Ladder High Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

ExcelBand Enhanced 3-color Regular Range Protein Marker, 250 μl x 2 ExcelBand 增强型三彩蛋白质常规分子量标记，用于15%蛋白质电泳凝胶分析，内含10条参考蛋白条带，经电泳分离后分别呈现蓝色、绿色和红色，条带分明，清晰可见，其中绿色条带为25KDa、红色条带为75KDa。片段范围：10-180KDa。4℃保存不小于3个月，-20℃保存不小于24个月。A ready-to-use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 180 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). PM2510 ExcelBand Enhanced 3-color Regular Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

ExcelBand All Blue Regular Range Protein Marker, 250 μl x 2 ExcelBand 湛蓝蛋白质常规分子量标记，用于15%蛋白质电泳凝胶分析，内含10条预染蓝色之蛋白质条带，条带分明，清晰可见，其中有两条加量参考条带，分别为25KDa和72KDa。片段范围：10-180KDa。4℃保存不小于3个月，-20℃保存不小于24个月。A blue protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-Glycine buffer). The PM1500 ExcelBand All Blue Regular Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

预染蛋白Marker，可在11-190kDa之间形成11条带。1750ul

预染蛋白Marker，可直接显色，条带宽，11-190kDa，350ul

预染蛋白marker。25ul。范围：1.7-42kDa纯化蛋白的混合物。蓝色,绿色或橙色的6条带横跨1.7至42 kDa。染料结合到蛋白质会影响在SDS-PAGE凝胶电泳中的移动。用法：1、在室温下解冻蛋白，不煮。2、轻轻地涡旋溶液,以确保混合均匀。3、每条道上样适量的蛋白Marker,如:Mini-Gel: 0.75 -1.0毫米厚:上样10μl 1.5毫米厚:上样20μl ——大的凝胶: 0.75 -1.0毫米厚:上样20μl 1.5毫米厚:上样40μl 4、未使用的蛋白储存于-20ºC以利长期保存。 Color-coded Prestained Protein Marker, Low Range (1.7-42 kDa)，25ul

预染蛋白marker，范围：43-315kDa，可形成蓝色,绿色，橙色或粉色八条带。25ul。用法：1.在室温下解冻蛋白，不煮。2.轻轻地涡旋溶液,以确保混合均匀。3.每条道上样适量的蛋白Marker:-Mini-Gel: 0.75 -1.0毫米厚:上样10μl 1.5毫米厚:上样20μl 大的凝胶:0.75 -1.0毫米厚:上样20μl 1.5毫米厚:上样40μl 4.未使用的蛋白储存于-20ºC以利长期保存。 Color-coded Prestained Protein Marker, High Range (43-315 kDa)，25ul

预染蛋白marker。250ul。范围：1.7-42kDa。纯化蛋白偶联染料复合物。电泳时可形成蓝色,绿色或橙色的6条带横跨1.7至42 kDa。染料结合到蛋白质会影响在SDS-PAGE凝胶电泳中的移动。用法：1、在室温下解冻蛋白，不煮。2、轻轻地涡旋溶液,以确保混合均匀。3、每条道上样适量的蛋白Marker:Mini-Gel: 0.75 -1.0毫米厚:上样10μl 1.5毫米厚:上样20μl ——大的凝胶: 0.75 -1.0毫米厚:上样20μl 1.5毫米厚:上样40μl 4、未使用的蛋白储存于-20ºC以利长期保存。 Color-coded Prestained Protein Marker, Low Range (1.7-42 kDa), 250ul

预染蛋白marker，范围：43-315kDa，可形成蓝色,绿色，橙色或粉色八条带。250ul。用法：1.在室温下解冻蛋白，不煮。2.轻轻地涡旋溶液,以确保混合均匀。3.每条道上样适量的蛋白Marker:-Mini-Gel: 0.75 -1.0毫米厚:上样10μl 1.5毫米厚:上样20μl 大的凝胶:0.75 -1.0毫米厚:上样20μl 1.5毫米厚:上样40μl 4.未使用的蛋白储存于-20ºC以利长期保存。 Color-coded Prestained Protein Marker, High Range (43-315 kDa), 250ul

用胰蛋白酶酶切牛血清白蛋白（BSA）生成多肽复杂混合物，再用碘乙酰亚胺（Iodoacetimide）还原和烷基化（CAM 修饰）复合物。该多肽混合物可用于校准 MALDI-TOF（基质辅助激光解吸/离子化飞行时间质谱仪）或 ESI（电喷雾电离）质谱（TOF、Q-TOF 或离子阱）。

Recommended use is 1-5 µl per lane in SDS-PAGEeach vial contains 500 µl volumeprestained molecular weight standards are offered for use in Western blotting applicationsunstained standard sizes: 200 kDa, 97 kDa, 66 kDa, 44 kDa