Monarch凝胶溶解缓冲液专为 Monarch DNA凝胶提取试剂盒 (T1020S/L)使用而设计,该缓冲液用来溶解包含目标DNA的琼脂糖;也可用于随后DNA吸附于纯化柱上的洗脱。Monarch Gel Dissolving Buffer is designed for use with the Monarch DNA Gel Extraction Kit (T1020S/L). This is the buffer used to dissolve the agarose containing the target DNA. The buffer also conditions the DNA for subsequent binding to the column.
DNA纯化和胶回收试剂盒,超高性价比,优秀的实验表现,独特的环保设计 The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving you a step. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging
DNA纯化和胶回收试剂盒,超高性价比,优秀的实验表现,独特的环保设计 The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving you a step. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging
胶回收与PCR 产物回收纯化二合为一,高效回收大于50bp的片段。回收率高,洗脱体积小(15uL),从而可获得高浓度的DNA回收产物。适用于TAE,TBE等各种胶缓冲系统。10分钟完成,省时省力。适用于PCR 产物纯化;从凝胶中抽提DNA 样本;浓缩反应产物以及去除盐离子;可以纯化浓缩单链DNA (需要NTC 缓冲液);可以去除样本中SDS 杂质(需要NTB 缓冲液)。纯化后的产物适用于:克隆、测序、PCR、限制酶内切等实验硅胶膜技术 微型离心柱样本 回收片段大小 50bp – 15 kbp回收效率 70 – 95 %A260/A280: 1.8 – 1.9洗脱体积: 15 – 50 μL制备时间 10 min / 6 preps载量 25μ gPCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume • Two applications in one kit, one buffer with optimal performance for both applications• High recoveries for fragments down to 50 bp• Minimized elution volume: 15 μL – highly concentrated DNA• Binding buffer with pH indicator• Separate buffers for single stranded DNA or SDS containing samples available• Suitable for all gel buffer systems (e.g., TAE, TBE)• Also available for QIAcube® (please see "Ordering information" and contact Technical Service at [email protected] for more information). Technology: Silica-membrane technology Format: Mini spin columns Sample material: Fragment size: 50 bp–approx. 20 kbp Typical recovery: 70–95% A260/A280: 1.8–1.9 Elution volume 15–30 µL Preparation time 10 min/6 preps Binding capacity 25 µg Applications • Purification of PCR products• Extraction of DNA from agarose gels• NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as:- nucleotides, primers- enzymes- mineral oil- PCR additives (e.g., salts, betaine, DMSO)- detergents (e.g., Tween 20, Triton X-100)- dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain) • Support protocol for DNA extraction from polyacrylamide gels• Support protocol for clean-up of single stranded DNA and support protocol for RNA extraction from agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”)• Support protocol for clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the kit, see “Ordering information”) • Typical downstream applications: cloning, sequencing, PCR, restriction analysis NucleoSpin® Gel and PCR Clean-up Columns,Collection Tubes (2 mL), buffers 50 preps for PCR clean-up and gel extraction - NucleoSpin Gel and PCR Clean-up Columns, Collection Tubes, buffers
胶回收与PCR 产物回收纯化二合为一,高效回收大于50bp的片段。回收率高,洗脱体积小(15uL),从而可获得高浓度的DNA回收产物。适用于TAE,TBE等各种胶缓冲系统。10分钟完成,省时省力。适用于PCR 产物纯化;从凝胶中抽提DNA 样本;浓缩反应产物以及去除盐离子;可以纯化浓缩单链DNA (需要NTC 缓冲液);可以去除样本中SDS 杂质(需要NTB 缓冲液)。纯化后的产物适用于:克隆、测序、PCR、限制酶内切等实验硅胶膜技术 微型离心柱样本 回收片段大小 50bp – 15 kbp回收效率 70 – 95 %A260/A280: 1.8 – 1.9洗脱体积: 15 – 50 μL制备时间 10 min / 6 preps载量 25μ gPCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume • Two applications in one kit, one buffer with optimal performance for both applications• High recoveries for fragments down to 50 bp• Minimized elution volume: 15 μL – highly concentrated DNA• Binding buffer with pH indicator• Separate buffers for single stranded DNA or SDS containing samples available• Suitable for all gel buffer systems (e.g., TAE, TBE)• Also available for QIAcube® (please see "Ordering information" and contact Technical Service at [email protected] for more information). Technology: Silica-membrane technology Format: Mini spin columns Sample material: Fragment size: 50 bp–approx. 20 kbp Typical recovery: 70–95% A260/A280: 1.8–1.9 Elution volume 15–30 µL Preparation time 10 min/6 preps Binding capacity 25 µg Applications • Purification of PCR products• Extraction of DNA from agarose gels• NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as:- nucleotides, primers- enzymes- mineral oil- PCR additives (e.g., salts, betaine, DMSO)- detergents (e.g., Tween 20, Triton X-100)- dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain) • Support protocol for DNA extraction from polyacrylamide gels• Support protocol for clean-up of single stranded DNA and support protocol for RNA extraction from agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”)• Support protocol for clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the kit, see “Ordering information”) • Typical downstream applications: cloning, sequencing, PCR, restriction analysis NucleoSpin® Gel and PCR Clean-up Columns,Collection Tubes (2 mL), buffers 250 preps for PCR-clean-up and gel extraction - NucleoSpin and PCR Clean-up Columns, Collection Tubes, buffers
胶回收与PCR 产物回收纯化二合为一,高效回收大于50bp的片段。回收率高,洗脱体积小(15uL),从而可获得高浓度的DNA回收产物。适用于TAE,TBE等各种胶缓冲系统。10分钟完成,省时省力。适用于PCR 产物纯化;从凝胶中抽提DNA 样本;浓缩反应产物以及去除盐离子;可以纯化浓缩单链DNA (需要NTC 缓冲液);可以去除样本中SDS 杂质(需要NTB 缓冲液)。纯化后的产物适用于:克隆、测序、PCR、限制酶内切等实验硅胶膜技术 微型离心柱样本 回收片段大小 50bp – 15 kbp回收效率 70 – 95 %A260/A280: 1.8 – 1.9洗脱体积: 15 – 50 μL制备时间 10 min / 6 preps载量 25μ gPCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume • Two applications in one kit, one buffer with optimal performance for both applications• High recoveries for fragments down to 50 bp• Minimized elution volume: 15 μL – highly concentrated DNA• Binding buffer with pH indicator• Separate buffers for single stranded DNA or SDS containing samples available• Suitable for all gel buffer systems (e.g., TAE, TBE)• Also available for QIAcube® (please see "Ordering information" and contact Technical Service at [email protected] for more information). Technology: Silica-membrane technology Format: Mini spin columns Sample material: Fragment size: 50 bp–approx. 20 kbp Typical recovery: 70–95% A260/A280: 1.8–1.9 Elution volume 15–30 µL Preparation time 10 min/6 preps Binding capacity 25 µg Applications • Purification of PCR products• Extraction of DNA from agarose gels• NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as:- nucleotides, primers- enzymes- mineral oil- PCR additives (e.g., salts, betaine, DMSO)- detergents (e.g., Tween 20, Triton X-100)- dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain) • Support protocol for DNA extraction from polyacrylamide gels• Support protocol for clean-up of single stranded DNA and support protocol for RNA extraction from agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”)• Support protocol for clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the kit, see “Ordering information”) • Typical downstream applications: cloning, sequencing, PCR, restriction analysis NucleoSpin® Gel and PCR Clean-up Columns,Collection Tubes (2 mL), buffers 10 preps for PCR clean-up and gel extraction - NucleoSpin Gel and PCR Clean-up Columns, Collection Tubes, buffers