即用型一次性色谱柱专用于快速、有效地从小分子中分离出小分子（>72 个碱基）未标记的或放射性标记的RNA。快速旋转柱专为低速、摆动式离心机而设计。

可和Biomics生产的 T7 sgRNA MICscript™ KIT配套使用。使用该试剂盒纯化后的sgRNA可直接进行转染及细胞显微注射。详细请联系4008513777.

方便快速地从血清和血浆中分离小分子RNA和DNA. 样品体积300ul，重复上样900ulIsolation of small RNA and DNA from plasma and serum • Efficient and selective isolation of small RNA from plasma or serum samples• Superior recovery of small RNA > 15 nt• Optional on-column DNA digest for increased sensitivity in downstream applications• Simple and fast procedure – without phenol / chloroform Technology: Silica-membrane technology Format: Mini spin columns Sample material: 300 µL plasma or serum (up to 900 µL with multiple loading)Fragment size: Elution volume: 20–50 µL Preparation time: 40 min/10 preps (without rDNase digestion); 70 min/10 preps (with rDNase digestion)Binding capacity: 200 µg Applications** • Isolation of small RNA from plasma or serum for cancer research (e.g., reliable monitoring of clinical parameters)• Typical downstream applications: real-time qRT-PCR, chip hybridisations NucleoSpin® miRNA Columns, Collection Tubes(2 mL), buffers, RNase-free rDNase 50 preps for the isolation of small RNA and DNA from plasma - NucleoSpin miRNA Columns, Collection Tubes, buffers, RNase-free rDNase

方便快速地从血清和血浆中分离小分子RNA和DNA. 样品体积300ul，重复上样900ulIsolation of small RNA and DNA from plasma and serum • Efficient and selective isolation of small RNA from plasma or serum samples• Superior recovery of small RNA > 15 nt• Optional on-column DNA digest for increased sensitivity in downstream applications• Simple and fast procedure – without phenol / chloroform Technology: Silica-membrane technology Format: Mini spin columns Sample material: 300 µL plasma or serum (up to 900 µL with multiple loading)Fragment size: Elution volume: 20–50 µL Preparation time: 40 min/10 preps (without rDNase digestion); 70 min/10 preps (with rDNase digestion)Binding capacity: 200 µg Applications** • Isolation of small RNA from plasma or serum for cancer research (e.g., reliable monitoring of clinical parameters)• Typical downstream applications: real-time qRT-PCR, chip hybridisations NucleoSpin® miRNA Columns, Collection Tubes(2 mL), buffers, RNase-free rDNase 250 preps for the isolation of small RNA and DNA from plasma - NucleoSpin miRNA Columns, Collection Tubes, buffers, RNase-free rDNase

方便快速地从血清和血浆中分离小分子RNA和DNA. 样品体积300ul，重复上样900ulIsolation of small RNA and DNA from plasma and serum • Efficient and selective isolation of small RNA from plasma or serum samples• Superior recovery of small RNA > 15 nt• Optional on-column DNA digest for increased sensitivity in downstream applications• Simple and fast procedure – without phenol / chloroform Technology: Silica-membrane technology Format: Mini spin columns Sample material: 300 µL plasma or serum (up to 900 µL with multiple loading)Fragment size: Elution volume: 20–50 µL Preparation time: 40 min/10 preps (without rDNase digestion); 70 min/10 preps (with rDNase digestion)Binding capacity: 200 µg Applications** • Isolation of small RNA from plasma or serum for cancer research (e.g., reliable monitoring of clinical parameters)• Typical downstream applications: real-time qRT-PCR, chip hybridisations NucleoSpin® miRNA Columns, Collection Tubes(2 mL), buffers, RNase-free rDNase 10 preps for the isolation of small RNA and DNA from plasma - NucleoSpin miRNA Columns, Collection Tubes, buffers, RNase-free rDNase

方便快速地提取Parallel isolation of small and large RNA • RNA purification fractionated by size: Isolation of small RNA only ( Isolation of small RNA ( 200 b) in two separate fractions Isolation of total RNA (small and large RNA in one fraction)• Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis• Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform• NucleoSpin® Filters for efficient sample homogenization• rDNase for efficient on-column removal of genomic DNA Technology Silica-membrane technology Format Mini spin columns Sample material Fragment size: Small RNA: 200 b Typical yield: 10 µg small RNA, 95 µg large RNA from 10(7) HeLa cells Elution volume: 30–100 µL Preparation time: Binding capacity 200 µg Applications**• Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue, and in combination with phenol / chloroform (e.g., TRIzol®) lysis• Purification of siRNA and large dsRNA from DICER reactionsTypical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization. Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridizationNucleoSpin® RNA Columns, NucleoSpin® miRNA Columns, NucleoSpin® Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 50 preps for the isolation of small and large RNA - NucleoSpin RNA Columns, NucleoSpin miRNA Columns, NucleoSpin Filters, NucleoSpin Protein Removal Columns, Collection Tubes, buffers, RNase-free rDNase

方便快速地提取Parallel isolation of small and large RNA • RNA purification fractionated by size: Isolation of small RNA only ( Isolation of small RNA ( 200 b) in two separate fractions Isolation of total RNA (small and large RNA in one fraction)• Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis• Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform• NucleoSpin® Filters for efficient sample homogenization• rDNase for efficient on-column removal of genomic DNA Technology Silica-membrane technology Format Mini spin columns Sample material Fragment size: Small RNA: 200 b Typical yield: 10 µg small RNA, 95 µg large RNA from 10(7) HeLa cells Elution volume: 30–100 µL Preparation time: Binding capacity 200 µg Applications**• Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue, and in combination with phenol / chloroform (e.g., TRIzol®) lysis• Purification of siRNA and large dsRNA from DICER reactionsTypical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization. Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridizationNucleoSpin® RNA Columns, NucleoSpin® miRNA Columns, NucleoSpin® Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 250 preps for the isolation of small and large RNA - NucleoSpin RNA Columns, NucleoSpin miRNA Columns, NucleoSpin Filters, NucleoSpin Protein Removal Columns, Collection Tubes, buffers, RNase-free rDNase