应用范围：IP 反应种属：Human,Mouse

活化的Rap1蛋白检测试剂盒 Active Rap1 Detection Kit, 1Kit

Active Ras Detection试剂盒可以检测内源性的GTP-结合状态的（有活性的）Ras蛋白。活性Ras检测试剂盒提供了足以检测细胞中激活状态Ras GTPase的所有试剂。GST-Raf1-RBD融合蛋白能够结合GTP-结合的活性状态的Ras，可以被谷胱甘肽树脂免疫沉淀。使用Ras mouse mAb用Western方法检测Ras的活化水平。 30 rxns.Active Ras Detection Kit detects endogenous levels of GTP-bound (active) Ras as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. The Active Ras Detection Kit provides all reagents necessary for measuring activation of Ras GTPase in the cell. GST-Raf1-RBD fusion protein is used to bind the activated form of GTP-bound Ras, which can then be immunoprecipitated with glutathione resin. Ras activation levels are then determined in western using a Ras mouse mAb.

Active Rho Detection试剂盒可以检测内源性的GTP-bound (active) Rho。试剂盒提供了所有足以检测细胞中激活状态Rho GTPase的试剂。GST-Rhotekin-RBD融合蛋白用于与GTP-bound Rho的激活状态结合，随后可以被谷胱甘肽树脂免疫沉淀。Rho的激活水平随后使用一个Rho Rabbit 抗体通过western blot实验检测。 30 rxns。 Active Rho Detection Kit detects endogenous levels of GTP-bound (active) Rho as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. The Active Rho Detection Kit provides all reagents necessary for measuring activation of Rho GTPase in the cell. GST-Rhotekin-RBD fusion protein is used to bind the activated form of GTP-bound Rho, which can then be immunoprecipitated with glutathione resin. Rho activation levels are then determined by western blot using a Rho Rabbit Antibody.

应用范围：WB 反应种属：Human,Mouse

Active Cdc42 Detection试剂盒可以检测内源性的GTP-bound (active) Cdc42。试剂盒提供了所有足以检测细胞中激活状态Cdc42 GTPase的试剂。GST-PAK1-PBD融合蛋白用于与GTP-bound cdc42的激活状态结合，随后可以被谷胱甘肽树脂免疫沉淀。Cdc42的激活水平随后使用一个cdc42 Mouse mAb通过western blot实验检测。 30 rxns。 Active Cdc42 Detection Kit detects endogenous levels of GTP-bound (active) Cdc42 as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. The Active Cdc42 Detection Kit provides all reagents necessary for measuring activation of Cdc42 GTPase in the cell. GST-PAK1-PBD fusion protein is used to bind the activated form of GTP-bound Cdc42, which can then be immunoprecipitated with glutathione resin. Cdc42 activation levels are then determined by western blot using a Cdc42 Mouse mAb.

应用范围：WB 反应种属：Human,Mouse

Active Rac1 Detection试剂盒可以识别内源性GTP-bound（active）Rac1。 试剂盒提供了所有足以检测细胞中激活状态的Rac1 GTPase的试剂。GST-PAK1-PBD融合蛋白用以与激活状态的GTP-bound Rac1结合，随后通过谷胱甘肽树脂被免疫沉淀。Rac1的激活水平随后通过Rac1 Mouse mAb进行western blot分析以检测。 30 rxns。 Active Rac1 Detection Kit detects endogenous levels of GTP-bound (active) Rac1 as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. The Active Rac1 Detection Kit provides all reagents necessary for measuring activation of Rac1 GTPase in the cell. GST-PAK1-PBD fusion protein is used to bind the activated form of GTP-bound Rac1, which can then be immunoprecipitated with glutathione resin. Rac1 activation levels are then determined by western blot using a Rac1 Mouse mAb.