SensiFAST探针法定量PCR试剂盒(No-ROX,500x20ul反应),对DNA模板或者cDNA模板进行定量。试剂盒中含有定量PCRmix,方便配制反应体系,减少污染风险,快速、灵敏、适合多重定量 通用探针法实时定量PCR试剂盒,不带ROX,适合包括TaqMan®, Scorpions®和分子信标等探针检测。抗体介导的热启动技术减少非特异结合,提高专一性,快速高效灵敏。20ul反应体系,500次反应。适用于不需正参照的机型。 Fast, highly reproducible real-time PCR and has been validated on commonly used real-time PCR instruments. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that SensiFAST Probe Kit delivers fast, highly-specific and ultra-sensitive real-time PCR.
Highly specific - minimal non-specific activity leading to better efficiency and greater accuracy
Ultra-sensitive - perfect for difficult and low copy number samples
Efficient multiplexing - increasing throughput and conserving precious sample material
Fast - optimized for fast cycling conditions, ideal for high-throughput
Instrument Compatibility
Roche LightCycler® 480, LightCycler® Nano, Bio-Rad iCycler®, iQ®5, MyiQ™, Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q, Eppendorf Mastercycler®ep realplex, PCRmax Eco™, Takara Thermal Cycler Dice® (TP800), Analytik Jena qTOWER 2 and Techne Quantica®, PrimeQ. See product selection table.
Description
SensiFAST™ Probe No-ROX Kit has been designed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments. The kit has been formulated for use with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.
SensiFAST Probe Kit has been optimized for fast mode on fast qPCR instruments and fast cycling conditions on standard qPCR instruments. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that the SensiFAST Probe Kit delivers shorter run times. It is also is highly reproducible, highly-specific and ultra-sensitive (Fig. 1), allowing more samples to be run with the highest confidence, ideal for high-throughput assays.
The advanced buffer chemistry and enhancers also make SensiFAST Probe very sensitive and perfect for multiplexing (Fig. 2). SensiFAST Probe No-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including dNTPs, stabilizers and enhancers.
For expression analysis, SensiFAST cDNA Synthesis Kit is the perfect partner for SensiFAST Probe No-ROX Kit for very fast cDNA synthesis and real-time results, without compromising sensitivity or quality.<
SensiFAST Probe No-ROX is used with real-time PCR instruments that do not require a passive reference.