识别位点:GAGCT/C 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:- 热灭活温度:65°C 稀释液:A  粘端匹配的酶:BanII (GAGCT/C), BsiHKAI, Bsp1286I (GAGCT/C) 同裂酶:Ecl136II^, Eco53kI^, EcoICRI^, Psp124BI , SstI

识别位点:TAC/GTA 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:CpG Methylation: Blocked 热灭活温度:80°C 稀释液:A  粘端匹配的酶:- 同裂酶:BstSNI, Eco105I

识别位点:GCG/C 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:CpG Methylation: Blocked 热灭活温度:65°C 稀释液:A  粘端匹配的酶:- 同裂酶:AspLEI , BstHHI, CfoI, GlaI^, R9529^, HinP1I^, HspAI ^

识别位点:G/GTNACC 缓冲液:NEBuffer 3.1 反应温度:60°C 甲基化敏感性:- 热灭活温度:- 稀释液:A  粘端匹配的酶:- 同裂酶:BstPI, Eco065I, Eco91I, EcoO65I, PspEI

识别位点:C/TTAAG 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:- 热灭活温度:65°C 稀释液:A  粘端匹配的酶:- 同裂酶:BfrI , BspTI, Bst98I, BstAFI, MspCI, Vha464I

识别位点:C/TNAG 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:- 热灭活温度:65°C 稀释液:B  粘端匹配的酶:- 同裂酶:BstDEI, HpyF3I

识别位点:C/CCGGG 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:CpG Methylation: Impaired 热灭活温度:65°C 稀释液:A  粘端匹配的酶:AgeI, BsaWI, BspEI, BsrFI, NgoMIV, SgrAI，AvaI (C/CCGGG) 同裂酶:Cfr9I, PspAI , SmaI^, TspMI, XmaCI

识别位点:GA/TC，只有当识别位点被甲基化时，DpnI 才能切割。从 dam+ 菌株纯化而来的 DNA 才是 DpnI 切割的底物。对哺乳动物基因组 DNA CpG 甲基化敏感。 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:CpG Methylation: Blocked by Overlapping 热灭活温度:80°C 稀释液:B  粘端匹配的酶:- 同裂酶:BfuCI^, BscFI^, Bsp143I^, BssMI^, BstENII^, BstKTI^, BstMBI^, Kzo9I^, MalI^, MboI^, NdeII^, Sau3AI^

识别位点:C/TCGAG 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:CpG Methylation: Impaired 热灭活温度:65°C 稀释液:A  粘端匹配的酶:PspXI，TliI，SalI 同裂酶:BssHI, PaeR7I, Sfr274I, SlaI, StrI, TliI

识别位点:AA/CGTT 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:CpG Methylation: Blocked 热灭活温度:- 稀释液:B  粘端匹配的酶:AccI (GT/CGAC), AciI, ClaI, BstBI, HinP1I, HpaII, NarI, TaqαI 同裂酶:Psp1406I

识别位点:G/AATTC 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:CpG Methylation: Blocked by Some Combinations of Overlapping 热灭活温度:65°C 稀释液:C  粘端匹配的酶:- 同裂酶:-

识别位点:A/AGCTT 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:- 热灭活温度:80°C 稀释液:B  粘端匹配的酶:- 同裂酶:-

识别位点:G/GATCC 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:- 热灭活温度:- 稀释液:A  粘端匹配的酶:BclI, DpnII；BglII, BstYI (R/GATCY)，BstYI (G/GATCC) 同裂酶:-

T4RNA连接酶反应缓冲液套装

识别位点:GGTAC/C 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:- 热灭活温度:- 稀释液:A  粘端匹配的酶:- 同裂酶:Acc65I^, Asp718I ^

识别位点:GGTAC/C 缓冲液:NEBuffer 1.1 反应温度:37°C 甲基化敏感性:- 热灭活温度:- 稀释液:A  粘端匹配的酶:- 同裂酶:Acc65I^, Asp718I ^

识别位点:C/YCGRG 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:CpG Methylation: Blocked 热灭活温度:80°C 稀释液:A  粘端匹配的酶:AvaI(C/CCGGG)-AgeI, BsaWI, BspEI, BsrFI (R/CCGGY), NgoMIV, SgrAI (CR/CCGGYG)； AvaI(C/TCGAG)-XhoI，SalI； AvaI(C/CCGGG)-XmaI 同裂酶:Ama87I , BcoI , BmeT110I, BsiHKCI, BsoBI, Eco88I, NspIII

识别位点:G/GWCC 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:dcm & CpG Methylation: Blocked by Overlapping 热灭活温度:80°C 稀释液:A  粘端匹配的酶:PpuMI (RG/GACCY，RG/GTCCY)，RsrII 同裂酶:AflI , Bme18I, Eco47I, PspO3I^, SinI, Vpak11AI^

识别位点:G/CTAGC 缓冲液:NEBuffer 2.1 反应温度:37°C 甲基化敏感性:CpG Methylation: Blocked by Some Combinations of Overlapping 热灭活温度:65°C 稀释液:C  粘端匹配的酶:AvrII, SpeI, StyI (C/CTAGG), XbaI 同裂酶:AsuNHI , BmtI^, BspOI^

识别位点:GA/TC，只有当识别位点被甲基化时，DpnI 才能切割。从 dam+ 菌株纯化而来的 DNA 才是 DpnI 切割的底物。对哺乳动物基因组 DNA CpG 甲基化敏感。 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:CpG Methylation: Blocked by Overlapping 热灭活温度:80°C 稀释液:B  粘端匹配的酶:- 同裂酶:BfuCI^, BscFI^, Bsp143I^, BssMI^, BstENII^, BstKTI^, BstMBI^, Kzo9I^, MalI^, MboI^, NdeII^, Sau3AI^

识别位点:TAGGGATAACAGGGTAAT(-9/-13) 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:- 热灭活温度:65°C 稀释液:B  粘端匹配的酶:- 同裂酶:-

识别位点:TGGCAAACAGCTATTATGGGTATTATGGGT(-13/-17) 缓冲液:NEBuffer PI-PspI + BSA 反应温度:65°C 甲基化敏感性:- 热灭活温度:- 稀释液:B  粘端匹配的酶:- 同裂酶:-

识别位点:ATCTATGTCGGGTGCGGAGAAAGAGGTAAT(-15/-19) 缓冲液:NEBuffer PI-SceI + BSA 反应温度:37°C 甲基化敏感性:- 热灭活温度:65°C 稀释液:B  粘端匹配的酶:- 同裂酶:-

识别位点:C/TCGAG 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:CpG Methylation: Impaired 热灭活温度:65°C 稀释液:A  粘端匹配的酶:PspXI，TliI，SalI 同裂酶:BssHI, PaeR7I, Sfr274I, SlaI, StrI, TliI

识别位点:C/TYRAG 缓冲液:CutSmart Buffer 反应温度:55°C 甲基化敏感性:- 热灭活温度:- 稀释液:A  粘端匹配的酶:- 同裂酶:-

识别位点:TG/CA 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:- 热灭活温度:65°C 稀释液:A  粘端匹配的酶:- 同裂酶:CviRI

识别位点:C/CNNGG 缓冲液:CutSmart Buffer 反应温度:60°C 甲基化敏感性:- 热灭活温度:80°C 稀释液:A  粘端匹配的酶:- 同裂酶:BseDI, BssECI, SecI

识别位点:A/CCGGT 缓冲液:CutSmart Buffer 反应温度:37°C 甲基化敏感性:CpG Methylation: Blocked 热灭活温度:65°C 稀释液:A  粘端匹配的酶:AvaI (C/CCGGG), XmaI，BsaWI, BspEI，BsrFI (A/CCGGT), SgrAI (CA/CCGGTG)，NgoMIV 同裂酶:AsiAI , BshTI, CspAI, PinAI

识别位点:/GATC 甲基化敏感性:对 dam 甲基化敏感。 缓冲液:NEBuffer DpnII 反应温度:37°C 热灭活温度:65°C 稀释液:B  粘端匹配的酶:BamHI, BclI, BglII, BstYI (R/GATCY)，MboI，Sau3AI 同裂酶:BfuCI, BscFI, Bsp143I, BssMI, BstENII, BstKTI^, BstMBI, DpnI^, Kzo9I, MalI^, MboI, NdeII^, Sau3AI