For fast purification of poly(A) mRNA from total RNA • High binding capacity: 5 μg poly(A) mRNA/mg oligo(dT) latex beads (20 μL bead suspension) • Support protocol for direct mRNA isolation from cells available • High quality poly(A) mRNA without degradation and DNA contamination • Convenient and fast processing by microcentrifugation using NucleoTrap® Microfilters • mRNA purification in only 30 min • Available in mini and midi format
Technology Affinity chromatography Format Oligo(dT) latex-bead suspension Sample material 250 µg total RNA Fragment size 50 b–20 kbp Typical yield 10 µg mRNA A260/A280 1.9–2.1 Elution volume 10–20 µL Preparation time 30 min/6 preps Binding capacity 5 µg poly(A) mRNA/20 µL oligo(dT) latex-bead suspension Applications* • Poly(A) mRNA isolation from total RNA • Clean-up of in-vitro transcripts • Direct purification of poly(A) mRNA from cells (support protocol) NucleoTrap® Microfilters, Microcentrifuge Tubes (2 mL),
Oligo(dT) Latex Beads, buffers 12 preps for the isolation of mRNA - NucleoTrap Microfilters, Microcentrifuge Tubes, NucleoTrap Oligo(dT) Latex Beads, buffers
For fast purification of poly(A) mRNA from total RNA • High binding capacity: 5 μg poly(A) mRNA/mg oligo(dT) latex beads (20 μL bead suspension) • Support protocol for direct mRNA isolation from cells available • High quality poly(A) mRNA without degradation and DNA contamination • Convenient and fast processing by microcentrifugation using NucleoTrap® Microfilters • mRNA purification in only 30 min • Available in mini and midi format
Technology Affinity chromatography Format Oligo(dT) latex-bead suspension Sample material: 1000 µg total RNA Fragment size 50 b–20 kbp Typical yield:40 µg mRNA A260/A280 1.9–2.1 Elution volume 10–20 µL Preparation time 30 min/6 preps Binding capacity 5 µg poly(A) mRNA/20 µL oligo(dT) latex-bead suspension Applications* • Poly(A) mRNA isolation from total RNA • Clean-up of in-vitro transcripts • Direct purification of poly(A) mRNA from cells (support protocol) NucleoTrap® Microfilters, Microcentrifuge Tubes (2 mL),
Oligo(dT) Latex Beads, buffers 12 preps for the isolation of mRNA - NucleoTrap Microfilters, Microcentrifuge Tubes, NucleoTrap Oligo(dT) Latex Beads, buffers