该产品是能用于从Poly A区域中分离mRNA的亲和基质。这种基质包含有与纤维素珠共价交联的Oligo (dT)25。结合容量 >400 A260units/g

用于从细胞裂解液和组织中小规模分离mRNA的亲和基质。此分离是通过 Oligo d(T)25与大部分真核生物mRNA中携带的ploy(A)形成共价杂交耦联实现的。既可用于细胞裂解后mRNA的直接分离，也可用于从总RNA中进行第二轮纯化。磁珠分离技术还能放大体系并将分离后的mRNA终体积进行调整，从而得到较小体积的完整RNA 洗脱液，不需要再从洗脱液中沉淀 poly(A)+ 转录物。磁珠能再生3次，实验者可选择将分离到的mRNA洗脱或直接以结合mRNA上的d(T)25 DNA为引物来进行cDNA 第一链合成。1 mg Oligo d(T)25能结合10 μg poly(A)+ RNA。

通过spin离心柱（NEB #S1408）快速、简便从真核细胞总RNA中分离全长poly(A)+ mRNA。Oligo(dT)25-纤维素珠具有较强结合容量和超强结合力，是亲和层析 Ploy(A)+ RNA 的最佳选择，40 分钟即可获得完整的 mRNA。分离样品可来自各种真核细胞裂解液，或真菌、植物及动物组织的总 RNA。该试剂盒提供的试剂可满足分离、沉淀8个样品中的 Poly(A)+ RNA，每个样品总 RNA 的量可高达1mg。分离得到的RNA可用于体外翻译实验、cDNA 文库制备、Northern、消减杂交或差异显示分析等。

用于从细胞或组织中分离完整的poly(A)+RNA，不需酚和其它有机溶剂。将Oligo d(T)25与1 μm顺磁性磁珠耦联后，以此为固相支持物直接结合poly(A)+ RNA。可进行多个样本的分离，自动化高通量分离。磁性分离技术可得到较小体积的完整RNA洗脱液，不需要再从洗脱液中沉淀 poly(A)+ 转录物。因此在 1 小时内就能分离得到体现原始样品中mRNA分布特性的完整poly(A)+ RNA。Oligo d(T)25 磁珠可再生3次，实验者可选择将分离到的 mRNA 洗脱下来或直接以结合于mRNA上的dT DNA 为引物来进行cDNA 第一链合成。

VAHTSTM mRNA Capture Beads是偶联了Oligo (dT)的1 μm顺磁性微球，适合于从纯化的总RNA中分离poly (A)+ RNA。磁性分离技术可从小体积样品中分离出完整的mRNA，避免了mRNA沉淀的步骤。整个操作过程可在一小时内完成。

For fast purification of poly(A) mRNA from total RNA
• High binding capacity: 5 μg poly(A) mRNA/mg oligo(dT) latex beads (20 μL bead suspension)
• Support protocol for direct mRNA isolation from cells available
• High quality poly(A) mRNA without degradation and DNA contamination
• Convenient and fast processing by microcentrifugation using NucleoTrap® Microfilters
• mRNA purification in only 30 min
• Available in mini and midi format

Technology Affinity chromatography
Format Oligo(dT) latex-bead suspension
Sample material 250 µg total RNA
Fragment size 50 b–20 kbp
Typical yield 10 µg mRNA
A260/A280 1.9–2.1
Elution volume 10–20 µL
Preparation time 30 min/6 preps
Binding capacity 5 µg poly(A) mRNA/20 µL oligo(dT) latex-bead suspension
Applications*
• Poly(A) mRNA isolation from total RNA
• Clean-up of in-vitro transcripts
• Direct purification of poly(A) mRNA from cells (support protocol)
NucleoTrap® Microfilters, Microcentrifuge Tubes (2 mL), Oligo(dT) Latex Beads, buffers
12 preps for the isolation of mRNA - NucleoTrap Microfilters, Microcentrifuge Tubes, NucleoTrap Oligo(dT) Latex Beads, buffers

VAHTSTM mRNA Capture Beads是偶联了Oligo (dT)的1 μm顺磁性微球，适合于从纯化的总RNA中分离poly (A)+ RNA。磁性分离技术可从小体积样品中分离出完整的mRNA，避免了mRNA沉淀的步骤。整个操作过程可在一小时内完成。

For fast purification of poly(A) mRNA from total RNA
• High binding capacity: 5 μg poly(A) mRNA/mg oligo(dT) latex beads (20 μL bead suspension)
• Support protocol for direct mRNA isolation from cells available
• High quality poly(A) mRNA without degradation and DNA contamination
• Convenient and fast processing by microcentrifugation using NucleoTrap® Microfilters
• mRNA purification in only 30 min
• Available in mini and midi format

Technology Affinity chromatography
Format Oligo(dT) latex-bead suspension
Sample material： 1000 µg total RNA
Fragment size 50 b–20 kbp
Typical yield：40 µg mRNA
A260/A280 1.9–2.1
Elution volume 10–20 µL
Preparation time 30 min/6 preps
Binding capacity 5 µg poly(A) mRNA/20 µL oligo(dT) latex-bead suspension
Applications*
• Poly(A) mRNA isolation from total RNA
• Clean-up of in-vitro transcripts
• Direct purification of poly(A) mRNA from cells (support protocol)
NucleoTrap® Microfilters, Microcentrifuge Tubes (2 mL), Oligo(dT) Latex Beads, buffers
12 preps for the isolation of mRNA - NucleoTrap Microfilters, Microcentrifuge Tubes, NucleoTrap Oligo(dT) Latex Beads, buffers