Magnetic-bead based isolation of RNA from tissue and cell samples • One-tube procedure minimizes risk of cross-contamination • Small elution volumes ≥ 50 μL • Suitable for manual and automated processing • Recombinant DNase included • Reducing agent TCEP included (no β-mercaptoethanol necessary)
Technology Magnetic-bead technology Format Highly reactive superparamagnetic beads Processing Manual or automated Sample material < 20 mg tissue, 2 x 106 cells Typical yield < 30 µg Elution volume ≥ 50 µL Preparation time < 120 min/96 preps Binding capacity Approx. 0.3 μg/μL beads NucleoMag® B-Beads, buffers, RNase-free rDNase 1 x 96 preps for the purification of total RNA - NucleoMag B-Beads, Buffers, Reducing Agent TCEP, RNase-free rDNase
8联管平行提取组织或细胞的总RNA，节约时间。rDNase可有效去除DNA污染。 Isolation of total RNA in flexible 8-well strip format and for high throughput in approved 96-well format • Time-saving parallel isolation of total RNA • rDNase included for efficient removal of genomic DNA • Processing under vacuum or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination • RNA ready to use for any kind of enzymatic reaction • NucleoSpin® 8 / 96 RNA Core Kits: Kits with basic content focussed on automation platforms.
Technology Silica-membrane technology Sample material < 10(7) cultured cells (centrifugation); < 30 mg tissue (centrifugation); saliva (collected with Oragene®) Fragment size > 200 b Typical yield < 100 µg A260/A280 1.90–2.10 Typical RIN (RNA integrity number) > 9 (cells) / ≥ 7 (tissue) RNA ratio 28S/18S approx. 2.1 Typical concentration 50–200 ng/µL Elution volume 50–130 µL Preparation time 45 min/6 strips; 70 min/plate Binding capacity 100 µg NucleoSpin® RNA Binding Strips, MN Wash Plates, MN Square-well Blocks, Racks of Tube Strips, Elution Plate U-bottom, Self-adhering Foil, buffers, RNase-free rDNase 12 x 8 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA Binding Strips, MN Wash Plates, MN Square-well Blocks , Racks of Tube Strips, Elution Plate U-bottom, Self-adhering Foil, buffers, RNase-free rDNase
Isolation of total RNA from whole blood (human or animal) in flexible 8-well strip format or approved 96-well format • Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4 °C – complete processing at RT) • Compatible with common blood collection tubes and anticoagulants(e.g., EDTA, citrate, and heparin) • Suitable for fresh and frozen blood samples • rDNase included for efficient removal of genomic DNA • Processing under vacuum or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination
Technology Silica-membrane technology Processing Manual or automated, vacuum or centrifugation Sample material < 400 µL whole blood (fresh or frozen) Fragment size > 200 nt Typical yield 1–8 µg (400 µL blood) Typical RNA quality (A260/A280) 1.9–2.1 Elution volume 50–130 µL Preparation time: 60 min/6 strips 100 min/plate Binding capacity 100 µg Applications** • Manual or automated isolation of total RNA from fresh or frozen whole blood stabilized with, for example, EDTA, citrate, or heparin • Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology ** RNA yield strongly depends on the leukocyte number in each individual blood sample. NucleoSpin® RNA Blood Binding Strips, MN Wash Plates, Square-well Blocks, Racks of Tube Strips, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase, Liquid Proteinase K 12 x 8 preps for the isolation of RNA from blood - NucleoSpin RNA Blood Binding Strips, MN Wash Plates, Racks of Tube Strips, Square-well Blocks, Elution Plates U-bottom, Self-adhering Foils, buffers, RNase-free rDNase, Liquid Proteinase K
96孔板高通量平行提取组织或细胞的总RNA，节约时间。rDNase可有效去除DNA污染。单孔载量100ug Isolation of total RNA in flexible 8-well strip format and for high throughput in approved 96-well format • Time-saving parallel isolation of total RNA • rDNase included for efficient removal of genomic DNA • Processing under vacuum or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination • RNA ready to use for any kind of enzymatic reaction • NucleoSpin® 8 / 96 RNA Core Kits: Kits with basic content focussed on automation platforms.
Technology Silica-membrane technology Sample material < 10(7) cultured cells (centrifugation); < 30 mg tissue (centrifugation); saliva (collected with Oragene®) Fragment size > 200 b Typical yield < 100 µg A260/A280 1.90–2.10 Typical RIN (RNA integrity number) > 9 (cells) / ≥ 7 (tissue) RNA ratio 28S/18S approx. 2.1 Typical concentration 50–200 ng/µL Elution volume 50–130 µL Preparation time 45 min/6 strips; 70 min/plate Binding capacity 100 µg NucleoSpin® RNA Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Block Low, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase x 96 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA Binding Plates, MN Wash Plates, MN Square-well Blocks, Round-well Block Low , Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase
Magnetic-bead based isolation of RNA from tissue and cell samples • One-tube procedure minimizes risk of cross-contamination • Small elution volumes ≥ 50 μL • Suitable for manual and automated processing • Recombinant DNase included • Reducing agent TCEP included (no β-mercaptoethanol necessary)
Technology Magnetic-bead technology Format Highly reactive superparamagnetic beads Processing Manual or automated Sample material < 20 mg tissue, 2 x 106 cells Typical yield < 30 µg Elution volume ≥ 50 µL Preparation time < 120 min/96 preps Binding capacity Approx. 0.3 μg/μL beads NucleoMag® B-Beads, buffers, RNase-free rDNase 4 x 96 preps for the purification of total RNA - NucleoMag B-Beads, Buffers, RNase-free rDNase, Reducing Agent TCEP
Isolation of total RNA from whole blood (human or animal) in flexible 8-well strip format or approved 96-well format • Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4 °C – complete processing at RT) • Compatible with common blood collection tubes and anticoagulants(e.g., EDTA, citrate, and heparin) • Suitable for fresh and frozen blood samples • rDNase included for efficient removal of genomic DNA • Processing under vacuum or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination
Technology Silica-membrane technology Processing Manual or automated, vacuum or centrifugation Sample material < 400 µL whole blood (fresh or frozen) Fragment size > 200 nt Typical yield 1–8 µg (400 µL blood) Typical RNA quality (A260/A280) 1.9–2.1 Elution volume 50–130 µL Preparation time: 60 min/6 strips 100 min/plate Binding capacity 100 µg Applications** • Manual or automated isolation of total RNA from fresh or frozen whole blood stabilized with, for example, EDTA, citrate, or heparin • Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology ** RNA yield strongly depends on the leukocyte number in each individual blood sample. NucleoSpin® RNA Blood Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Block Low, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase, Liquid Proteinase K 2 x 96 preps for the isolation of total RNA from blood - NucleoSpin RNA Blood Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Blocks Low, Elution Plates U-bottom, Self-adhering Foils, buffers, RNase-free rDNase, Liquid Proteinase K
8联管平行提取组织或细胞的总RNA，节约时间。rDNase可有效去除DNA污染。 Isolation of total RNA in flexible 8-well strip format and for high throughput in approved 96-well format • Time-saving parallel isolation of total RNA • rDNase included for efficient removal of genomic DNA • Processing under vacuum or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination • RNA ready to use for any kind of enzymatic reaction • NucleoSpin® 8 / 96 RNA Core Kits: Kits with basic content focussed on automation platforms.
Technology Silica-membrane technology Sample material < 10(7) cultured cells (centrifugation); < 30 mg tissue (centrifugation); saliva (collected with Oragene®) Fragment size > 200 b Typical yield < 100 µg A260/A280 1.90–2.10 Typical RIN (RNA integrity number) > 9 (cells) / ≥ 7 (tissue) RNA ratio 28S/18S approx. 2.1 Typical concentration 50–200 ng/µL Elution volume 50–130 µL Preparation time 45 min/6 strips; 70 min/plate Binding capacity 100 µg NucleoSpin® RNA Binding Strips, buffers, RNase-free rDNase 48 x 8 preps for the isolation of total RNA from cells and tissue - NucleoSpin RNA Binding Strips, buffers, RNase-free rDNase
96孔板高通量平行提取组织或细胞的总RNA，节约时间。rDNase可有效去除DNA污染。单孔载量100ug Isolation of total RNA in flexible 8-well strip format and for high throughput in approved 96-well format • Time-saving parallel isolation of total RNA • rDNase included for efficient removal of genomic DNA • Processing under vacuum or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination • RNA ready to use for any kind of enzymatic reaction • NucleoSpin® 8 / 96 RNA Core Kits: Kits with basic content focussed on automation platforms.
Technology Silica-membrane technology Sample material < 10(7) cultured cells (centrifugation); < 30 mg tissue (centrifugation); saliva (collected with Oragene®) Fragment size > 200 b Typical yield < 100 µg A260/A280 1.90–2.10 Typical RIN (RNA integrity number) > 9 (cells) / ≥ 7 (tissue) RNA ratio 28S/18S approx. 2.1 Typical concentration 50–200 ng/µL Elution volume 50–130 µL Preparation time 45 min/6 strips; 70 min/plate Binding capacity 100 µg NucleoSpin® RNA Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Block Low, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase x 96 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA Binding Plates, MN Wash Plates MN Square-well Blocks, Elution Plates U-bottom, Self-adhering Foils, Round-well Block Low, buffers, RNase-free rDNase
Isolation of total RNA from whole blood (human or animal) in flexible 8-well strip format or approved 96-well format • Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4 °C – complete processing at RT) • Compatible with common blood collection tubes and anticoagulants(e.g., EDTA, citrate, and heparin) • Suitable for fresh and frozen blood samples • rDNase included for efficient removal of genomic DNA • Processing under vacuum or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination
Technology Silica-membrane technology Processing Manual or automated, vacuum or centrifugation Sample material < 400 µL whole blood (fresh or frozen) Fragment size > 200 nt Typical yield 1–8 µg (400 µL blood) Typical RNA quality (A260/A280) 1.9–2.1 Elution volume 50–130 µL Preparation time: 60 min/6 strips 100 min/plate Binding capacity 100 µg Applications** • Manual or automated isolation of total RNA from fresh or frozen whole blood stabilized with, for example, EDTA, citrate, or heparin • Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology ** RNA yield strongly depends on the leukocyte number in each individual blood sample. NucleoSpin® RNA Blood Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Block Low, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase, Liquid Proteinase K 4 x 96 preps for the isolation of total RNA from blood - NucleoSpin RNA Blood Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Blocks Low, Elution Plates U-bottom, Self-adhering Foils, buffers, RNase-free rDNase, Liquid Proteinase K
8联管平行提取组织或细胞的总RNA，节约时间。rDNase可有效去除DNA污染。 Isolation of total RNA in flexible 8-well strip format and for high throughput in approved 96-well format • Time-saving parallel isolation of total RNA • rDNase included for efficient removal of genomic DNA • Processing under vacuum or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination • RNA ready to use for any kind of enzymatic reaction • NucleoSpin® 8 / 96 RNA Core Kits: Kits with basic content focussed on automation platforms.
Technology Silica-membrane technology Sample material < 10(7) cultured cells (centrifugation); < 30 mg tissue (centrifugation); saliva (collected with Oragene®) Fragment size > 200 b Typical yield < 100 µg A260/A280 1.90–2.10 Typical RIN (RNA integrity number) > 9 (cells) / ≥ 7 (tissue) RNA ratio 28S/18S approx. 2.1 Typical concentration 50–200 ng/µL Elution volume 50–130 µL Preparation time 45 min/6 strips; 70 min/plate Binding capacity 100 µg NucleoSpin® RNA Binding Strips, MN Wash Plates, MN Square-well Blocks, Racks of Tube Strips, Elution Plate U-bottom, Self-adhering Foil, buffers, RNase-free rDNase 60 x 8 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA Binding Strips, MN Wash Plates, MN Square-well Blocks, Racks of Tube Strips, Elution Plates U-bottom, Self-adhering Foils, buffers, RNase-free rDNase
Isolation of total RNA from whole blood (human or animal) in flexible 8-well strip format or approved 96-well format • Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4 °C – complete processing at RT) • Compatible with common blood collection tubes and anticoagulants(e.g., EDTA, citrate, and heparin) • Suitable for fresh and frozen blood samples • rDNase included for efficient removal of genomic DNA • Processing under vacuum or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination
Technology Silica-membrane technology Processing Manual or automated, vacuum or centrifugation Sample material < 400 µL whole blood (fresh or frozen) Fragment size > 200 nt Typical yield 1–8 µg (400 µL blood) Typical RNA quality (A260/A280) 1.9–2.1 Elution volume 50–130 µL Preparation time: 60 min/6 strips 100 min/plate Binding capacity 100 µg Applications** • Manual or automated isolation of total RNA from fresh or frozen whole blood stabilized with, for example, EDTA, citrate, or heparin • Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology ** RNA yield strongly depends on the leukocyte number in each individual blood sample. NucleoSpin® RNA Blood Binding Strips, MN Wash Plates, Square-well Blocks, Racks of Tube Strips, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase, Liquid Proteinase K 60 x 8 preps for the isolation of RNA from blood - NucleoSpin RNA Blood Binding Strips, MN Wash Plates, Racks of Tube Strips, Square-well Blocks, Elution Plates U-bottom, Self-adhering Foils, buffers, RNase-free rDNase, Liquid Proteinase K
96孔板高通量平行提取组织或细胞的总RNA，节约时间。rDNase可有效去除DNA污染。单孔载量100ug Isolation of total RNA in flexible 8-well strip format and for high throughput in approved 96-well format • Time-saving parallel isolation of total RNA • rDNase included for efficient removal of genomic DNA • Processing under vacuum or by centrifugation • Suitable for manual and automated processing • Innovative MN Wash Plate minimizes risk of cross-contamination • RNA ready to use for any kind of enzymatic reaction • NucleoSpin® 8 / 96 RNA Core Kits: Kits with basic content focussed on automation platforms.
Technology Silica-membrane technology Sample material < 10(7) cultured cells (centrifugation); < 30 mg tissue (centrifugation); saliva (collected with Oragene®) Fragment size > 200 b Typical yield < 100 µg A260/A280 1.90–2.10 Typical RIN (RNA integrity number) > 9 (cells) / ≥ 7 (tissue) RNA ratio 28S/18S approx. 2.1 Typical concentration 50–200 ng/µL Elution volume 50–130 µL Preparation time 45 min/6 strips; 70 min/plate Binding capacity 100 µg NucleoSpin® RNA Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Block Low, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase 24 x 96 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA Binding Plates, MN Wash Plates, MN Square-well Blocks, Round-well Block Low , Elution Plates U-bottom, Self-adhering Foils, buffers, RNase-free rDNas
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