第二代阴离子交换技术，适于纯化转染级别纯度的高分子量载体如P1, BACs, PACs, 产量可达150ug，仅需75分钟。Column filter可同步完成裂解上清液分离和DNA吸附。 Purification of P1, BACs, PACs, and other large constructs from E. coli. Transfection-grade plasmid DNA. • Optimized column design – one prep in approximately 75 min • Column filter included – parallel lysate clearing and loading onto the column • Optimal silica material – yields up to 150 μg • Transfection-grade plasmid DNA using proven anion-exchange technology • LyseControl for visualization of a completed lysis / neutralization Technology： Anion-exchange chromatography Format： Gravity flow columns Lysate clarification： Column filters Sample material： 250–750 mL E. coli culture Vector size： < 300 kbp Typical yield： 10–150 µg A260/A280： 1.80–1.95 Preparation time： 75 min/2–4 preps Binding capacity： 150 µg NucleoBond® Xtra BAC Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A 10 preps for the isolation of low-copy large construct plasmid DNA - NucleoBond Xtra BAC Columns, buffers, RNase
高通量纯化大型质粒和大载体如cosmids, BACs（< 250 kbp），测序级纯度。Time and cost efficient high-throughput purification of plasmid DNA, cosmids, BACs • Suitable for high-throughput purification of large constructs (cosmids, BACs) • Manual or automated processing Technology Alkaline lysis with subsequent filtration and precipitation Format 96-well plates Processing Manual or automated, vacuum or centrifugation (centrifuge required for precipitation) Lysate clarification 96-well filter plates Sample material 1.1–1.3 mL E. coli culture Vector size < 250 kbp Typical yield 8 µg from 1.1–1.3 mL E. coli culture (high-copy plasmid); <1 µg from 1.3–3.9 mL E. coli culture (BACs) Preparation time 90 min/2 plates NucleoSpin® Flash Filter Plates, MN Square-well Blocks, Square-well Blocks, Gas-permeable Foil, Self-adhering Foil, buffers, RNase A 2 x 96 preps for the isolation of plasmids and large constructs - NucleoSpin Flash Filter Plates, MN Square-well Blocks, Square-well Blocks, Gas-permeable Foils, Self-adhering Foils, buffers, RNase A
第二代阴离子交换技术，适于纯化转染级别纯度的高分子量载体如P1, BACs, PACs, 产量可达150ug，仅需75分钟。Column filter可同步完成裂解上清液分离和DNA吸附。 Purification of P1, BACs, PACs, and other large constructs from E. coli. Transfection-grade plasmid DNA. • Optimized column design – one prep in approximately 75 min • Column filter included – parallel lysate clearing and loading onto the column • Optimal silica material – yields up to 150 μg • Transfection-grade plasmid DNA using proven anion-exchange technology • LyseControl for visualization of a completed lysis / neutralization Technology： Anion-exchange chromatography Format： Gravity flow columns Lysate clarification： Column filters Sample material： 250–750 mL E. coli culture Vector size： < 300 kbp Typical yield： 10–150 µg A260/A280： 1.80–1.95 Preparation time： 75 min/2–4 preps Binding capacity： 150 µg NucleoBond® Xtra BAC Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A 25 preps for the isolation of low-copy larg construct plasmid DNA - NucleoBond Xtra BAC Columns, buffers, RNase A
高通量纯化大型质粒和大载体如cosmids, BACs（< 250 kbp），测序级纯度。Time and cost efficient high-throughput purification of plasmid DNA, cosmids, BACs • Suitable for high-throughput purification of large constructs (cosmids, BACs) • Manual or automated processing Technology Alkaline lysis with subsequent filtration and precipitation Format 96-well plates Processing Manual or automated, vacuum or centrifugation (centrifuge required for precipitation) Lysate clarification 96-well filter plates Sample material 1.1–1.3 mL E. coli culture Vector size < 250 kbp Typical yield 8 µg from 1.1–1.3 mL E. coli culture (high-copy plasmid); <1 µg from 1.3–3.9 mL E. coli culture (BACs) Preparation time 90 min/2 plates NucleoSpin® Flash Filter Plates, MN Square-well Blocks, Square-well Blocks, Gas-permeable Foil, Self-adhering Foil, buffers, RNase A 4 x 96 preps for the isolation of plasmids and large constructs - NucleoSpin Flash Filter Plates, MN Square-well Blocks, Square-well Blocks, Gas-permeable Foils, Self-adhering Foils, buffers, RNase A
高通量纯化大型质粒和大载体如cosmids, BACs（< 250 kbp），测序级纯度。Time and cost efficient high-throughput purification of plasmid DNA, cosmids, BACs • Suitable for high-throughput purification of large constructs (cosmids, BACs) • Manual or automated processing Technology Alkaline lysis with subsequent filtration and precipitation Format 96-well plates Processing Manual or automated, vacuum or centrifugation (centrifuge required for precipitation) Lysate clarification 96-well filter plates Sample material 1.1–1.3 mL E. coli culture Vector size < 250 kbp Typical yield 8 µg from 1.1–1.3 mL E. coli culture (high-copy plasmid); <1 µg from 1.3–3.9 mL E. coli culture (BACs) Preparation time 90 min/2 plates NucleoSpin® Flash Filter Plates, MN Square-well Blocks, Square-well Blocks, Gas-permeable Foil, Self-adhering Foil, buffers, RNase A 24 x 96 preps for the isolation of plasmids and large constructs - NucleoSpin Flash Filter Plates, MN Square-well Blocks, Square-well Blocks, Gas-permeable Foils, Self-adhering Foils, buffers, RNase A
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