快捷灵敏简便的蛋白定量试剂,仅需40分钟 Fast, sensitive, and convenient assay for protein quantification • Highest sensitivity and flexibility • Reducing agent and detergent compatible • Fast procedure allows protein quantification in only 40 min
Sample size: < 600 µL containing 0.6–200 µg protein (BSA equivalents) Protein concentration: Approx. 10–20,000 ng/µL Sample type: Protein solved in Protein Solving Buffer PSB, PLB, Laemmli buffer, or equivalent, preferable free of nucleic acids Correlation coefficient: 0.97–1.00 Wavelength for light extinction measurement: 570 nm (530–700 nm) Time Approx. 40 min
Applications • Protein quantification assays in microplates, microcuvettes, semimicrocuvettes, low-volume photometer (e.g., NanoDrop™) • Protein quantification in Laemmli buffer • Protein quantification in buffers containing up to 10% SDS • Protein quantification in buffers containing reducing agents (e.g., β-mercaptoethanol (715 mM), TCEP (50 mM), or DTT) • Protein quantification in buffers containing bromophenol blue (e.g., 0.02%) Buffer PSB, Quantification Reagent, BSA reagents for the protein quantification of 50 samples and 6 calibration curves with 7 calibration points each - Buffer PSB, Quantification Reagent, BSA
快捷灵敏简便的蛋白定量试剂,仅需40分钟 Fast, sensitive, and convenient assay for protein quantification • Highest sensitivity and flexibility • Reducing agent and detergent compatible • Fast procedure allows protein quantification in only 40 min
Sample size: < 600 µL containing 0.6–200 µg protein (BSA equivalents) Protein concentration: Approx. 10–20,000 ng/µL Sample type: Protein solved in Protein Solving Buffer PSB, PLB, Laemmli buffer, or equivalent, preferable free of nucleic acids Correlation coefficient: 0.97–1.00 Wavelength for light extinction measurement: 570 nm (530–700 nm) Time Approx. 40 min
Applications • Protein quantification assays in microplates, microcuvettes, semimicrocuvettes, low-volume photometer (e.g., NanoDrop™) • Protein quantification in Laemmli buffer • Protein quantification in buffers containing up to 10% SDS • Protein quantification in buffers containing reducing agents (e.g., β-mercaptoethanol (715 mM), TCEP (50 mM), or DTT) • Protein quantification in buffers containing bromophenol blue (e.g., 0.02%) Buffer PSB, Quantification Reagent, BSA reagents for the protein quantification of 250 samples and 25 calibration curves with 7 calibration points each - Buffer PSB, Quantification Reagent, BSA