BCA蛋白分析试剂盒，可用于检测总蛋白含量。
BCA Protein Assay Kit, 1Kit

快捷灵敏简便的蛋白定量试剂，仅需40分钟
Fast, sensitive, and convenient assay for protein quantification
• Highest sensitivity and flexibility
• Reducing agent and detergent compatible
• Fast procedure allows protein quantification in only 40 min

Sample size: < 600 µL containing 0.6–200 µg protein (BSA equivalents)
Protein concentration: Approx. 10–20,000 ng/µL
Sample type: Protein solved in Protein Solving Buffer PSB, PLB, Laemmli buffer, or equivalent, preferable free of nucleic acids
Correlation coefficient: 0.97–1.00
Wavelength for light extinction measurement: 570 nm (530–700 nm)
Time Approx. 40 min

Applications
• Protein quantification assays in microplates, microcuvettes, semimicrocuvettes, low-volume photometer (e.g., NanoDrop™)
• Protein quantification in Laemmli buffer
• Protein quantification in buffers containing up to 10% SDS
• Protein quantification in buffers containing reducing agents (e.g., β-mercaptoethanol (715 mM), TCEP (50 mM), or DTT)
• Protein quantification in buffers containing bromophenol blue (e.g., 0.02%)
Buffer PSB, Quantification Reagent, BSA
reagents for the protein quantification of 50 samples and 6 calibration curves with 7 calibration points each - Buffer PSB, Quantification Reagent, BSA

快捷灵敏简便的蛋白定量试剂，仅需40分钟
Fast, sensitive, and convenient assay for protein quantification
• Highest sensitivity and flexibility
• Reducing agent and detergent compatible
• Fast procedure allows protein quantification in only 40 min

Sample size: < 600 µL containing 0.6–200 µg protein (BSA equivalents)
Protein concentration: Approx. 10–20,000 ng/µL
Sample type: Protein solved in Protein Solving Buffer PSB, PLB, Laemmli buffer, or equivalent, preferable free of nucleic acids
Correlation coefficient: 0.97–1.00
Wavelength for light extinction measurement: 570 nm (530–700 nm)
Time Approx. 40 min

Applications
• Protein quantification assays in microplates, microcuvettes, semimicrocuvettes, low-volume photometer (e.g., NanoDrop™)
• Protein quantification in Laemmli buffer
• Protein quantification in buffers containing up to 10% SDS
• Protein quantification in buffers containing reducing agents (e.g., β-mercaptoethanol (715 mM), TCEP (50 mM), or DTT)
• Protein quantification in buffers containing bromophenol blue (e.g., 0.02%)
Buffer PSB, Quantification Reagent, BSA
reagents for the protein quantification of 250 samples and 25 calibration curves with 7 calibration points each - Buffer PSB, Quantification Reagent, BSA

Bradford 法( 考马斯亮蓝法)，是目前灵敏度更高的蛋白质浓度测定方法之一。当Bradford 染色液( 考马斯亮蓝G250) 和蛋白在酸性条件下结合时，溶液颜色由棕黑色转为蓝色，更大吸光值波长由456 nm 转为595 nm，吸光值与蛋白质的含量在一定浓度范围内有较好的线性关系。通过测定吸光值大小并对照标准蛋白的吸光值，推算出蛋白浓度，实现了蛋白浓度测定的快速，稳定和高灵敏度。Bradford 染色液为2 倍浓缩母液，便于储存。

Bradford 法( 考马斯亮蓝法)，是目前灵敏度更高的蛋白质浓度测定方法之一。当Bradford 染色液( 考马斯亮蓝G250) 和蛋白在酸性条件下结合时，溶液颜色由棕黑色转为蓝色，更大吸光值波长由456 nm 转为595 nm，吸光值与蛋白质的含量在一定浓度范围内有较好的线性关系。通过测定吸光值大小并对照标准蛋白的吸光值，推算出蛋白浓度，实现了蛋白浓度测定的快速，稳定和高灵敏度。Bradford 染色液为2 倍浓缩母液，便于储存。

BCA 蛋白浓度测定试剂盒（BCA Protein Quantification Kit），是目前灵敏度更高的蛋白质测定方法之一。在碱性条件下，蛋白质将Cu2+还原为Cu+，Cu+ 与独特的BCA Reagent A（含有BCA）相互作用产生敏感的颜色反应，形成紫色的络合物。该水溶性的复合物在A562 nm 处显示强烈的吸光性，吸光度和蛋白质浓度在广泛范围内有良好的线性关系，根据吸光值可以推算出蛋白浓度。因此，使用酶标仪测定其在A562 nm 处的吸收值，并与标准曲线对比，即可计算待测蛋白的浓度。 常用浓度的去垢剂SDS，Triton X-100，Tween-20等不影响检测结果， 但受螯合剂(EDTA，EGTA)、还原剂（DTT，巯基乙醇）和脂类的影响。实验中，若发现样品稀释液或裂解液本身背景值较高，可使用Bradford 蛋白浓度测定试剂盒。

BCA 蛋白浓度测定试剂盒（BCA Protein Quantification Kit），是目前灵敏度更高的蛋白质测定方法之一。在碱性条件下，蛋白质将Cu2+还原为Cu+，Cu+ 与独特的BCA Reagent A（含有BCA）相互作用产生敏感的颜色反应，形成紫色的络合物。该水溶性的复合物在A562 nm 处显示强烈的吸光性，吸光度和蛋白质浓度在广泛范围内有良好的线性关系，根据吸光值可以推算出蛋白浓度。因此，使用酶标仪测定其在A562 nm 处的吸收值，并与标准曲线对比，即可计算待测蛋白的浓度。 常用浓度的去垢剂SDS，Triton X-100，Tween-20等不影响检测结果， 但受螯合剂(EDTA，EGTA)、还原剂（DTT，巯基乙醇）和脂类的影响。实验中，若发现样品稀释液或裂解液本身背景值较高，可使用Bradford 蛋白浓度测定试剂盒。